DocumentCode :
46750
Title :
Surface-Enhanced Raman Scattering Spectroscopy for the Detection of Glutamate and \\gamma -Aminobutyric Acid in Serum by Partial Least Squares Analysis
Author :
Monfared, Ali Momenpour T. ; Tiwari, Vidhu S. ; Trudeau, Vance L. ; Anis, Hanan
Author_Institution :
Sch. of Electr. Eng. & Comput. Sci., Univ. of Ottawa, Ottawa, ON, Canada
Volume :
7
Issue :
3
fYear :
2015
fDate :
Jun-15
Firstpage :
1
Lastpage :
16
Abstract :
Glutamate (GLU) and γ-aminobutyric acid (GABA) are important amino acid neurotransmitters that, respectively, mediate rapid excitatory and inhibitory neurotransmission in the brain. Serum GLU and GABA levels could play a significant role in pathogenesis of the encephalopathic state, early detection of autism, and the neurobiology of depression. The optimal concentration ratio of GLU and GABA is vital for neuroendocrine control, and deviations in their synthesis are linked to epilepsy. Additionally, circulating GLU affects GABA levels in striatal extracellular fluid. The present work explores the feasibility of employing surface-enhanced Raman scattering spectroscopy, in conjunction with partial least squares (PLS) , for simultaneous measurement of physiological concentrations of GLU and GABA in their aqueous solution and blood serum. A PLS calibration model showed a high coefficient of determination (R2 >0.98) between the spectral data of GLU and GABA and their respective concentrations. The low root mean square error of prediction (RMSEP) value was ~2.7 μM, and it established the accuracy of the calibration model. The present methodology enabled the detection of GLU and GABA as low as 8 μM. This provides a cost-effective alternative to existing techniques for quantification of neurotransmitters.
Keywords :
brain; calibration; least squares approximations; neurophysiology; surface enhanced Raman scattering; GABA optimal concentration ratio; GABA physiological concentration; GLU optimal concentration ratio; GLU physiological concentration; PLS calibration model; amino acid neurotransmitter; autism detection; blood serum; brain; depression neurobiology; encephalopathic state; epilepsy; gamma-aminobutyric acid; glutamate detection; neuroendocrine control; neurotransmission; neurotransmitter quantification; partial least squares analysis; serum GABA level; serum GLU level; striatal extracellular fluid; surface-enhanced Raman scattering spectroscopy; Amino acids; Blood; Detectors; Fluids; Nanoparticles; Neurotransmitters; Raman scattering; GABA; GLU; PLS; Serum; serum; surface enhanced Raman scattering (SERS); surface-enhanced Raman scattering (SERS);
fLanguage :
English
Journal_Title :
Photonics Journal, IEEE
Publisher :
ieee
ISSN :
1943-0655
Type :
jour
DOI :
10.1109/JPHOT.2015.2423284
Filename :
7096928
Link To Document :
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