Title :
Automated Extended Volume Imaging of Tissue using Confocal and Optical Microscopy
Author :
Sands, G.B. ; Gerneke, D.A. ; Smaill, B.H. ; Grice, I. J Le
Author_Institution :
Bioeng. Inst., Auckland Univ.
fDate :
Aug. 30 2006-Sept. 3 2006
Abstract :
Conventional histologic techniques cannot readily be used for 3D reconstruction of large tissue volumes. We have developed an imaging rig which supports both confocal and light microscopy, and utilizes a surface imaging approach to serially image embedded tissue blocks while maintaining alignment and registration of the image series
Keywords :
biological tissues; biomedical equipment; biomedical optical imaging; image registration; optical microscopy; automated extended tissue volume imaging; confocal microscopy; image alignment; image registration; image series; imaging rig; optical microscopy; surface imaging approach; Biomedical optical imaging; Brain; Digital cameras; Ethanol; Heart; Image resolution; Optical filters; Optical imaging; Optical microscopy; Resins;
Conference_Titel :
Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE
Conference_Location :
New York, NY
Print_ISBN :
1-4244-0032-5
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2006.259723