DocumentCode :
471467
Title :
Fibroblastic Differentiation of Human Mesenchymal Stem Cells using Connective Tissue Growth Factor
Author :
Lee, Chang H. ; Moioli, Eduardo K. ; Mao, Jeremy J.
Author_Institution :
Dept. of Orthodontics, Illinois Univ., Chicago, IL
fYear :
2006
fDate :
Aug. 30 2006-Sept. 3 2006
Firstpage :
775
Lastpage :
778
Abstract :
The present study was designed to explore an ex vivo culturing protocol for fibroblastic differentiation of human mesenchymal stem cells (hMSCs) using connective tissue growth factor (CTGF). Fibroblastic differentiation from stem cells is of widespread significance in the engineering of virtually all tissues including tendons, ligaments, periodontal ligament, cranial sutures and as interstitial filler of all organs. The treatment with 100 ng/ml of recombinant human CTGF and 50 mug/ml ascorbic acids on monolayer cultured hMSCs showed significant increases in type I collagen and tenascin-C (Tn-C) contents by 2 and 4 wks. In addition, CTGF-treated hMSCs failed to show osteogenic or chondrogenic differentiation. The present data show that CTGF is an effective induction factor for fibroblastic differentiation of hMSCs. These findings have implications for engineering fibrous tissue by providing the initial evidence of a reproducible protocol for fibroblastic differentiation of hMSCs
Keywords :
biochemistry; cellular biophysics; molecular biophysics; proteins; tissue engineering; ascorbic acids; chondrogenic differentiation; cranial sutures; ex vivo fibroblastic differentiation; fibrous tissue engineering; human mesenchymal stem cells; interstitial filler; osteogenic differentiation; periodontal ligament; recombinant human connective tissue growth factor; tenascin-C; tendons; type I collagen; Biomedical engineering; Bones; Connective tissue; Cranial; Fibroblasts; Humans; Ligaments; Protocols; Stem cells; Tendons;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology Society, 2006. EMBS '06. 28th Annual International Conference of the IEEE
Conference_Location :
New York, NY
ISSN :
1557-170X
Print_ISBN :
1-4244-0032-5
Electronic_ISBN :
1557-170X
Type :
conf
DOI :
10.1109/IEMBS.2006.259866
Filename :
4461866
Link To Document :
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