Title :
Surface Functionalization for Protein Microarray
Author :
Simion, Monica ; Ruta, Lavinia ; Kleps, Irina ; Mihailescu, Carmen ; Ignat, Teodora ; Stan, Dana ; Craciunoiu, Florin ; Miu, Mihaela ; Bragaru, Adina
Author_Institution :
Nat. Inst. for Reserch & Dev. in Microtechnologies (IMT), Bucharest
fDate :
Oct. 15 2007-Sept. 17 2007
Abstract :
This paper presents an attempt to use two substrate types silicon and glass for microarray technique, in order to find the best chemistry surface for immobilization of proteins in microarray technology. Different chemical substrate were studied (glass slides and silicon slides coated with gold and poly-L-lysine) printed with BSA and marked with CY3. The slides were printed and after that washed with PBS (pH=7.4) 20, 40 and 60 minutes. The intensity of the fluorescent signal of the spots before and after washing was analyzed using Gene TAC Analyser (Genomic Solutions) and the UV spectra of the washing residues were recorded.
Keywords :
bio-optics; fluorescence; molecular biophysics; proteins; surface chemistry; ultraviolet spectra; Gene TAC Analyser; Si-Au; SiO2-Au; UV spectra; bovine serum albumin; fluorescent signal intensity; glass substrate; gold; pH; poly-L-lysine; protein microarray; silicon substrate; surface chemistry; surface functionalization; time 20 min; time 40 min; time 60 min; washing residues; Bioinformatics; Chemical technology; Chemistry; Fluorescence; Genomics; Glass; Gold; Proteins; Signal analysis; Silicon; protein microarray; surface functionalisation;
Conference_Titel :
Semiconductor Conference, 2007. CAS 2007. International
Conference_Location :
Sinaia
Print_ISBN :
978-1-4244-0847-4
DOI :
10.1109/SMICND.2007.4519665
