Investigation of Liposome based Chondroitin Sulphate Effects on Human Chondrocites

Author

Trif, M. ; Moldovan, L. ; Zarnescu, O.

Author_Institution

Inst. of Biochem., Romanian Acad., Bucharest

Volume

1

fYear

2007

fDate

Oct. 15 2007-Sept. 17 2007

Firstpage

151

Lastpage

154

Abstract

The purpose of this study was to establish the most efficient liposome-chondroitin sulphate (CS) formulation for experiments in human chondrocytes (HC) cell culture. Comparative studies using CS, empty liposomes and liposome-CS systems were performed in order to evaluate their effect on growth and morphology of HC after 24 and 48 hours of culture. Results from MTT and light microscopy indicated that CS, empty liposomes and liposome-CS systems do not induced appreciable cytotoxic effects and cells maintain normal morphology when compared to control HC.

Keywords

bone; cellular biophysics; diseases; lipid bilayers; optical microscopy; orthopaedics; patient treatment; toxicology; MTT assay; cell morphology; cell proliferation; cytotoxic effects; empty liposomes; human chondrocytes cell culture; inflammatory disorders; light microscopy; lipid-based carrier systems; liposome based chondroitin sulphate effects; liposome-CS formulation; liposome-CS systems; rheumatic disorders; Biochemistry; Biology; Cells (biology); Chemical analysis; Drugs; Humans; Lipidomics; Morphology; Research and development; Solvents; cell proliferation; chondroitin sulphate; human chodrocytes; inflammation; liposomes;

fLanguage

English

Publisher

ieee

Conference_Titel

Semiconductor Conference, 2007. CAS 2007. International

Conference_Location

Sinaia

ISSN

1545-827X

Print_ISBN

978-1-4244-0847-4

Type

conf

DOI

10.1109/SMICND.2007.4519669

Filename

4519669