Title :
Investigation of Liposome based Chondroitin Sulphate Effects on Human Chondrocites
Author :
Trif, M. ; Moldovan, L. ; Zarnescu, O.
Author_Institution :
Inst. of Biochem., Romanian Acad., Bucharest
fDate :
Oct. 15 2007-Sept. 17 2007
Abstract :
The purpose of this study was to establish the most efficient liposome-chondroitin sulphate (CS) formulation for experiments in human chondrocytes (HC) cell culture. Comparative studies using CS, empty liposomes and liposome-CS systems were performed in order to evaluate their effect on growth and morphology of HC after 24 and 48 hours of culture. Results from MTT and light microscopy indicated that CS, empty liposomes and liposome-CS systems do not induced appreciable cytotoxic effects and cells maintain normal morphology when compared to control HC.
Keywords :
bone; cellular biophysics; diseases; lipid bilayers; optical microscopy; orthopaedics; patient treatment; toxicology; MTT assay; cell morphology; cell proliferation; cytotoxic effects; empty liposomes; human chondrocytes cell culture; inflammatory disorders; light microscopy; lipid-based carrier systems; liposome based chondroitin sulphate effects; liposome-CS formulation; liposome-CS systems; rheumatic disorders; Biochemistry; Biology; Cells (biology); Chemical analysis; Drugs; Humans; Lipidomics; Morphology; Research and development; Solvents; cell proliferation; chondroitin sulphate; human chodrocytes; inflammation; liposomes;
Conference_Titel :
Semiconductor Conference, 2007. CAS 2007. International
Conference_Location :
Sinaia
Print_ISBN :
978-1-4244-0847-4
DOI :
10.1109/SMICND.2007.4519669
