Title :
Three-dimensional super-resolution single-molecule fluorescence imaging using a double-helix point spread function
Author :
Thompson, Michael A. ; Biteen, Julie S. ; Moerner, W.E. ; Pavani, Sri Rama Prasanna ; Piestun, Rafael
Author_Institution :
Dept. of Chem., Stanford Univ., Stanford, CA, USA
Abstract :
Fluorescence imaging with resolution ten times better than the diffraction limit in three dimensions over a depth of field of 2 mum is demonstrated with a widefield microscope that exhibits a double-helix point spread function.
Keywords :
fluorescence; image resolution; light diffraction; optical images; optical microscopy; optical transfer function; 3D superresolution; depth 2 mum; diffraction limit; double-helix point spread function; fluorescence microscopy; optical transfer function; single-molecule fluorescence imaging; widefield microscopy; Biomedical optical imaging; Fluorescence; Image resolution; Lenses; Optical diffraction; Optical imaging; Optical microscopy; Signal resolution; Spatial resolution; Transfer functions; (100.6640) Superresolution; (110.4850) Optical transfer functions; (180.6900) Three-dimensional microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
