Title :
Background-free, 3D vibrational imaging by stimulated Raman scattering microscopy
Author :
Ozeki, Yasuyuki ; Dake, Fumihiro ; Kajiyama, Shin´ichiro ; Fukui, Kiichi ; Itoh, Kazuyoshi
Author_Institution :
Dept. of Mater. & Life Sci., Osaka Univ., Suita, Japan
Abstract :
We propose and experimentally show that stimulated Raman scattering can realize novel 3D nonlinear-optical Raman microscopy that is immune to nonresonant background signals. We demonstrate high-contrast molecular imaging of polystyrene beads and plant cells.
Keywords :
bio-optics; biological techniques; nonlinear optics; optical microscopy; stimulated Raman scattering; 3D vibrational imaging; high-contrast molecular imaging; nonlinear-optical Raman microscopy; nonresonant background signal; plant cell imaging; polystyrene bead; stimulated Raman scattering microscopy; Biological information theory; Frequency; Intensity modulation; Microscopy; Optical scattering; Optical sensors; Phase modulation; Pulse modulation; Raman scattering; Resonance; (180.5655) Raman microscopy; (190.2640) Stimulated scattering, modulation, etc.;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
