Title :
3D fluorescent particle tracking with nanometer scale accuracies using a double-helix point spread function
Author :
Pavani, Sri Rama Prasanna ; Piestun, Rafael
Author_Institution :
Dept. of Electr. & Comput. Eng., Univ. of Colorado at Boulder, Boulder, CO, USA
Abstract :
We demonstrate parallel three-dimensional (3D) tracking of multiple fluorescent microspheres with nanometer scale accuracies by engineering the 3D point spread function of a wide-field microscope to present a double-helix along the optical axis.
Keywords :
fluorescence; nanophotonics; optical microscopy; optical tracking; optical transfer function; particle tracks; 3D double-helix point spread function; multiple fluorescent microsphere; nanometer scale; parallel 3D particle tracking; wide-field microscope; Biomedical optical imaging; Fluorescence; Focusing; Nanobioscience; Optical imaging; Optical microscopy; Optical modulation; Optical scattering; Optical signal processing; Particle tracking; (110.1758) Computational imaging; (180.6900) Three-dimensional microscopy; (280.7250) Velocimetry;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
