Title :
Label-free optical detection of biomolecular reaction kinetics in microarray format for high-throughput screening applications
Author :
Sun, Y.S. ; Landry, J.P. ; Fei, Y.Y. ; Luo, J.T. ; Lam, K.S. ; Zhu, X.D.
Author_Institution :
Dept. of Phys., Univ. of California at Davis, Davis, CA, USA
Abstract :
Using a combination of an oblique-incidence reflectivity difference (OI-RD) scanning microscope and a customized 8-chamber sample cartridge, we detect 300 surface-immobilized molecular targets reacting with up to 8 different analytes simultaneously on a single slide.
Keywords :
molecular biophysics; optical microscopy; 8-chamber sample cartridge; biomolecular reaction kinetics; high-throughput screening; label-free optical detection; microarray format; oblique-incidence reflectivity difference scanning microscope; surface-immobilized molecular targets; Biomedical optical imaging; Fluorescence; Glass; Kinetic theory; Labeling; Laser beams; Microscopy; Optical detectors; Probes; Reflectivity; 170.0170; 180.0180; 240.0310; 310.0310;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
