Title :
Three-dimensional nanoscopy of biological samples
Author :
Vaziri, Alipasha ; Tang, Jianyong ; Shroff, Hari ; Shank, Charles V.
Author_Institution :
Howard Hughes Med. Inst., Ashburn, VA, USA
Abstract :
We have demonstrated super-resolution imaging of protein distributions in cells at depth at multiple layers with a lateral localization precision better than 50 nm. The approach is based on combining photoactivated localization microscopy with temporal focusing.
Keywords :
bio-optics; cellular biophysics; nanobiotechnology; optical microscopy; proteins; 3d nanoscopy; biological samples; photoactivated localization microscopy; protein; superresolution imaging; temporal focusing; Biomedical optical imaging; Cells (biology); Fluorescence; Focusing; Image resolution; Nanobioscience; Optical imaging; Optical microscopy; Proteins; Signal resolution;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
