• DocumentCode
    500514
  • Title

    Three-dimensional nanoscopy of biological samples

  • Author

    Vaziri, Alipasha ; Tang, Jianyong ; Shroff, Hari ; Shank, Charles V.

  • Author_Institution
    Howard Hughes Med. Inst., Ashburn, VA, USA
  • fYear
    2009
  • fDate
    2-4 June 2009
  • Firstpage
    1
  • Lastpage
    2
  • Abstract
    We have demonstrated super-resolution imaging of protein distributions in cells at depth at multiple layers with a lateral localization precision better than 50 nm. The approach is based on combining photoactivated localization microscopy with temporal focusing.
  • Keywords
    bio-optics; cellular biophysics; nanobiotechnology; optical microscopy; proteins; 3d nanoscopy; biological samples; photoactivated localization microscopy; protein; superresolution imaging; temporal focusing; Biomedical optical imaging; Cells (biology); Fluorescence; Focusing; Image resolution; Nanobioscience; Optical imaging; Optical microscopy; Proteins; Signal resolution;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
  • Conference_Location
    Baltimore, MD
  • Print_ISBN
    978-1-55752-869-8
  • Electronic_ISBN
    978-1-55752-869-8
  • Type

    conf

  • Filename
    5225923