Title :
Measurement of two-photon excitation spectrum of various fluorophores with Fourier transform nonlinear spectroscopy
Author :
Hashimoto, Hiroshi ; Isobe, Keisuke ; Suda, Akira ; Kannari, Fumihiko ; Kawano, Hiroyuki ; Mizuno, Hideaki ; Miyawaki, Atsushi ; Midorikawa, Katsumi
Author_Institution :
Laser Technol. Lab., RIKEN Adv. Sci. Inst., Wako, Japan
Abstract :
We show a technique to measure the two-photon excitation spectrum of various fluorophores, based on Fourier transform nonlinear spectroscopy with the use of ultrabroadband laser (670-1100 nm).
Keywords :
Fourier transform spectroscopy; bio-optics; biological techniques; measurement by laser beam; nonlinear optics; proteins; two-photon spectra; Fourier transform nonlinear spectroscopy; fluorescent protein; fluorophores; two-photon absorption spectrum; two-photon excitation spectrum; ultrabroadband laser; wavelength 670 nm to 1100 nm; Fluorescence; Fourier transforms; High-resolution imaging; Laser excitation; Optical imaging; Optical microscopy; Proteins; Pulse modulation; Resonance; Spectroscopy; (170.2520) Fluorescence microscopy; (300.6420) Spectroscopy, nonlinear;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
