Title :
A femtosecond Yb:KGd(WO4)2 laser for biological imaging of yellow fluorescent protein
Author :
Sandkuijl, D. ; Major, A. ; Cisek, R. ; Grang, G. ; Mathur, J. ; Stewart, B. ; Barzda, V.
Author_Institution :
Dept. of Phys., Univ. of Toronto, Mississauga, ON, Canada
Abstract :
Real time multiphoton excitation fluorescence microscopy of enhanced yellow fluorescent protein was performed with low photobleaching using a femtosecond Yb:KGd(WO4)2 laser. Using a conventional Ti:S laser this was previously unfeasible due to low excitation efficiency.
Keywords :
biological techniques; fluorescence; gadolinium compounds; high-speed optical techniques; laser beam applications; molecular biophysics; multiphoton processes; optical microscopy; optical saturable absorption; potassium compounds; proteins; solid lasers; ytterbium; KGd(WO4)2:Yb; biological imaging; excitation efficiency; femtosecond laser; multiphoton excitation fluorescence microscopy; photobleaching; yellow fluorescent protein; Biomedical optical imaging; Fluorescence; Laser excitation; Nonlinear optics; Optical harmonic generation; Optical imaging; Optical microscopy; Optical pumping; Photobleaching; Ultrafast optics; (140.3615) Lasers, Ytterbiums; (180.4315) Nonlinear microscopy;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
