Title :
Fiber-optic multiphoton in vivo flow cytometry
Author :
Chang, Yu-Chung ; Ye, Jing Yong ; Thomas, Thommey ; Cao, Zhengyi ; Kotlyar, Alina ; Baker, James R., Jr. ; Norris, Theodore B.
Author_Institution :
Center for Ultrafast Opt. Sci., Univ. of Michigan, Ann Arbor, MI, USA
Abstract :
We demonstrate the use of a double-clad fiber probe to conduct two-photon excited flow cytometry in a live mouse. High detection efficiency of GFP-expressing cells is demonstrated, and the initial dynamics of injected circulating cells is observed.
Keywords :
biomedical measurement; blood; cancer; cellular biophysics; fibre optic sensors; fluorescence; molecular biophysics; optical fibre cladding; proteins; tumours; two-photon processes; blood stream; cancer; circulating tumor cells; detection efficiency; double-clad fiber probe; fiber-optic flow cytometry; green fluorescent protein-expressing cells; in vivo flow cytometry; injected circulating cells; multiphoton flow cytometry; two-photon excited flow cytometry; Biomedical optical imaging; Blood; Cells (biology); Fluorescence; In vivo; Optical fibers; Optical scattering; Optical sensors; Probes; Ultrafast optics; (060.2370) Fiber optics sensors; (170.3890) Medical optics instrumentation; (190.4180) Multiphoton processes;
Conference_Titel :
Lasers and Electro-Optics, 2009 and 2009 Conference on Quantum electronics and Laser Science Conference. CLEO/QELS 2009. Conference on
Conference_Location :
Baltimore, MD
Print_ISBN :
978-1-55752-869-8
Electronic_ISBN :
978-1-55752-869-8
