Title :
Cloning and Bioinformatics Analysis of an Endoglucanase 2 from Trichoderma reesei
Author :
Yang, Yanling ; Liu, Lihua ; Diao, Miao ; Lin, Zhiwei ; Guo, Wunian ; Wang, Shihua
Author_Institution :
Key Lab. of Biopesticide & Chem. Biol., Fujian Agric. & Forestry Univ., Fuzhou, China
Abstract :
Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using some online or offline services.
Keywords :
DNA; bioinformatics; cellular biophysics; encoding; microorganisms; sequences; DNA fragment encoding; EG 2 strain production; bioinformatics analysis; cloning analysis; endoglucanase; expression vector; offline service; online service; sequence; trichoderma reesei CBS368; vector pET28a; vector pMD18-T; Biochemistry; Bioinformatics; Capacitive sensors; Chemical analysis; Cloning; Encoding; Fungi; Laboratories; Microorganisms; Signal processing; Trichoderma reesei CBS368; bioinformatics; clone; expression;
Conference_Titel :
Software Engineering, 2009. WCSE '09. WRI World Congress on
Conference_Location :
Xiamen
Print_ISBN :
978-0-7695-3570-8
DOI :
10.1109/WCSE.2009.175
