Cloning and Bioinformatics Analysis of an Endoglucanase 2 from Trichoderma reesei

Author

Yang, Yanling ; Liu, Lihua ; Diao, Miao ; Lin, Zhiwei ; Guo, Wunian ; Wang, Shihua

Author_Institution

Key Lab. of Biopesticide & Chem. Biol., Fujian Agric. & Forestry Univ., Fuzhou, China

Volume

2

fYear

2009

fDate

19-21 May 2009

Firstpage

268

Lastpage

272

Abstract

Trichoderma reesei CBS368, a strong endoglucanase 2 (EG 2) producing strain, was isolated in our laboratory. The DNA fragment encoding the EG 2 was cloned in pMD18-T and sequenced. In order to achieve its expression, the vector pET28a (+) and pET32a (+) were used as expression vector, and the recombinant EG 2 was successfully expressed in E. coli BL21 (DE3). Bioinformatic analysis was conducted using some online or offline services.

Keywords

DNA; bioinformatics; cellular biophysics; encoding; microorganisms; sequences; DNA fragment encoding; EG 2 strain production; bioinformatics analysis; cloning analysis; endoglucanase; expression vector; offline service; online service; sequence; trichoderma reesei CBS368; vector pET28a; vector pMD18-T; Biochemistry; Bioinformatics; Capacitive sensors; Chemical analysis; Cloning; Encoding; Fungi; Laboratories; Microorganisms; Signal processing; Trichoderma reesei CBS368; bioinformatics; clone; expression;

fLanguage

English

Publisher

ieee

Conference_Titel

Software Engineering, 2009. WCSE '09. WRI World Congress on

Conference_Location

Xiamen

Print_ISBN

978-0-7695-3570-8

Type

conf

DOI

10.1109/WCSE.2009.175

Filename

5319668