Title :
A microfluidic system for semicontinuous bead-based biosensing platforms
Author :
Kim, Hye Won ; Kim, Young Man ; Yoo, Sung Keun ; Yoon, Sang Youl ; Lee, Jong Hyun ; Yang, Sung
Author_Institution :
Department of Mechatronics, 261 Cheomdan-gwagiro (Oryong-dong), Buk-gu, Gwangju, Lorea 500-712, Korea
Abstract :
Conventional bead-based micro immunoassays do not guarantee high reliability because of the working principle for the trapping methods which cause inconsistency in trapping of cytometric beads. This paper presents a new bead-based micro immunoassay system for multiple or semicontinuous assays with high reliability and accuracy using hole-patterned structures and double-cladding optical fibers. Trapping test showed selective trapping of beads and consistency of the number of the trapped beads over repeated tests. Immunoassay using IL-6 showed that the cytometric beads were trapped in ordered fashion and provided a detection limit on the order of pg/ml.
Keywords :
Biomedical optical imaging; Biosensors; Electron traps; Fluorescence; Immune system; Microfluidics; Optical fibers; Optical microscopy; Optical sensors; Testing;
Conference_Titel :
Communications and Photonics Conference and Exhibition (ACP), 2009 Asia
Conference_Location :
Shanghai, China
Print_ISBN :
978-1-55752-877-3
Electronic_ISBN :
978-1-55752-877-3