Title :
Force spectroscopy of single receptor-ligand bond using an optical trap
Author :
Zhang, Yan ; Sun, Ganyun ; Li, Ning ; Huo, Bo ; Lu, Shouqin ; Long, Mian
Author_Institution :
National Microgravity Laboratory and Center for Biomechanics and Bioengineering, Institute of Mechanics, Chinese Academy of Sciences, Beijing 100190, China
Abstract :
Here we developed an thermal fluctuation assay using optical trap technique, which enables to visualize consecutive binding-unbinding transition and to unravel the bond dissociation at low spring cosntant and low loading rate. This novel method provided further understandings in monitoring biophysics of receptor-ligand interactions.
Keywords :
Biomedical optical imaging; Biophysics; Bonding forces; Charge carrier processes; Fluctuations; Monitoring; Spectroscopy; Springs; Thermal loading; Visualization;
Conference_Titel :
Communications and Photonics Conference and Exhibition (ACP), 2009 Asia
Conference_Location :
Shanghai, China
Print_ISBN :
978-1-55752-877-3
Electronic_ISBN :
978-1-55752-877-3
