Title :
Monitoring cellular metabolism with fluorescence lifetime of reduced nicotinamide adenine dinucleotide
Author :
Ghukasyan, Vladimir V. ; Kao, Fu-Jen
Author_Institution :
Institute of Biophotonics, National Yang-Ming University, Taiwan
Abstract :
Fluorescence lifetime imaging has gained popularity as a sensitive technique to monitor the functional/conformational states of NADH - one of the main compounds of the oxidative phosphorylation. We hereby review the development and current application of cellular metabolism observation via NADH FLIM, illustrating it with examples of physiological and pathological states.
Keywords :
Biochemistry; Data analysis; Equations; Fluorescence; Mathematical model; Microscopy; Monitoring; Optical imaging; Pathology; Photonics;
Conference_Titel :
Communications and Photonics Conference and Exhibition (ACP), 2009 Asia
Conference_Location :
Shanghai, China
Print_ISBN :
978-1-55752-877-3
Electronic_ISBN :
978-1-55752-877-3
