Title :
Development of a polymeric device for gene expression profiling
Author :
Fanzio, Paola ; Angeli, E. ; Mussi, Valentina ; Valbusa, Ugo ; Rivolo, Paola ; Frascella, Francesca ; Pirri, Candido Fabrizio
Author_Institution :
Italian Inst. of Technol. (IIT), Genova, Italy
Abstract :
We report the description of a new low cost method to create a device based on a chemically modified nanopore created on a polymeric membrane. Using Soft-Lithography and Nanofabrication it can be possible to create a nanopore on a free standing polymeric membrane. After oligonucleotide functionalization of the lumen of the nanopore it is possible to detect specific sequences of DNA, thanks to the hybridization properties of the double helix. The sensing principle is based on transient interruptions in the ion-current induced by entry, transport and exit of a particular analyte from the pore.
Keywords :
DNA; bioelectric phenomena; biological techniques; biomembrane transport; biosensors; genetics; molecular biophysics; nanobiotechnology; nanofabrication; nanoporous materials; polymer films; soft lithography; DNA; biosensor; chemically modified nanopore; gene expression profiling; lumen; nanofabrication; oligonucleotide functionalization; polymeric device; polymeric membrane; sensing principle; soft-lithography; Biomembranes; Chemicals; Costs; DNA; Gene expression; Nanofabrication; Nanoporous materials; Polymers; Sequences; Transient analysis; nanofabrication; nanopore; polymeric device; ultra-thin membrane;
Conference_Titel :
Nanotechnology, 2009. IEEE-NANO 2009. 9th IEEE Conference on
Conference_Location :
Genoa
Print_ISBN :
978-1-4244-4832-6
Electronic_ISBN :
1944-9399
