Development of a polymeric device for gene expression profiling

Author

Fanzio, Paola ; Angeli, E. ; Mussi, Valentina ; Valbusa, Ugo ; Rivolo, Paola ; Frascella, Francesca ; Pirri, Candido Fabrizio

Author_Institution

Italian Inst. of Technol. (IIT), Genova, Italy

fYear

2009

fDate

26-30 July 2009

Firstpage

613

Lastpage

615

Abstract

We report the description of a new low cost method to create a device based on a chemically modified nanopore created on a polymeric membrane. Using Soft-Lithography and Nanofabrication it can be possible to create a nanopore on a free standing polymeric membrane. After oligonucleotide functionalization of the lumen of the nanopore it is possible to detect specific sequences of DNA, thanks to the hybridization properties of the double helix. The sensing principle is based on transient interruptions in the ion-current induced by entry, transport and exit of a particular analyte from the pore.

Keywords

DNA; bioelectric phenomena; biological techniques; biomembrane transport; biosensors; genetics; molecular biophysics; nanobiotechnology; nanofabrication; nanoporous materials; polymer films; soft lithography; DNA; biosensor; chemically modified nanopore; gene expression profiling; lumen; nanofabrication; oligonucleotide functionalization; polymeric device; polymeric membrane; sensing principle; soft-lithography; Biomembranes; Chemicals; Costs; DNA; Gene expression; Nanofabrication; Nanoporous materials; Polymers; Sequences; Transient analysis; nanofabrication; nanopore; polymeric device; ultra-thin membrane;

fLanguage

English

Publisher

ieee

Conference_Titel

Nanotechnology, 2009. IEEE-NANO 2009. 9th IEEE Conference on

Conference_Location

Genoa

ISSN

1944-9399

Print_ISBN

978-1-4244-4832-6

Electronic_ISBN

1944-9399

Type

conf

Filename

5394754