A microfluidic system for semicontinuous bead-based biosensing platforms

Conventional bead-based micro immunoassays do not guarantee high reliability and repeatability due to the trapping mechanisms of the systems which cause inconsistency in trapping of cytometric beads. This paper presents a new beadbased micro immunoassay system for semicontinuous assays with high reliability and accuracy in cytometric bead trapping by using the hole-patterned microstructures. Trapping test showed the assembly of single layer of bead array in ordered fashion. In addition that, the µ-immunoassay exhibits that the fluorescence intensity linearly increases in proportion to Biotin-4-Fluorescin (B4F) concentration and a 10 pg/mL appears to be the lowest detectable concentration for B4F after incubation time of 5 minutes.