DocumentCode :
519875
Title :
A segmentation method for microscope images of BY-2 tobacco cells in suspension cultures
Author :
Goclawski, Jaroslaw ; Sekulska-Nalewajko, Joanna ; Aniol, Patryk
Author_Institution :
Comput. Engeenering Dept., Tech. Univ. of Lodz, Lodz, Poland
fYear :
2010
fDate :
20-23 April 2010
Firstpage :
192
Lastpage :
196
Abstract :
This paper describes a segmentation method of BY-2 tobacco cells proceeded from suspension cultures. The method separates foreground objects by the detection of their boundaries and then eliminates cell clumps and artefacts based on their different inside brightness and skeleton structures. Single cell objects are restored by the thickening of binary cell residues remaining after morphological operation of cleaning the holes under the cell chain masks. Experimental results of the segmentation for a series of BY-2 images as well as cell enumeration and segmentation errors are presented.
Keywords :
cellular biophysics; image segmentation; medical image processing; microscopes; BY-2 tobacco cell; binary cell residue; cell clumps; foreground object; microscope image; morphological operation; segmentation method; single cell object; skeleton structure; suspension culture; Brightness; Histograms; Image restoration; Image segmentation; Lighting; Microscopy; Morphological operations; Object detection; Shape; Skeleton; BY-2 tobacco cells; Elliptical Fourier Descriptors; dilatation; hit-miss operation; image processing; morphological operations; segmentation; skeletoning;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Perspective Technologies and Methods in MEMS Design (MEMSTECH), 2010 Proceedings of VIth International Conference on
Conference_Location :
Lviv
Print_ISBN :
978-1-4244-7325-0
Electronic_ISBN :
978-966-2191-11-0
Type :
conf
Filename :
5499288
Link To Document :
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