Title :
A segmentation method for microscope images of BY-2 tobacco cells in suspension cultures
Author :
Goclawski, Jaroslaw ; Sekulska-Nalewajko, Joanna ; Aniol, Patryk
Author_Institution :
Comput. Engeenering Dept., Tech. Univ. of Lodz, Lodz, Poland
Abstract :
This paper describes a segmentation method of BY-2 tobacco cells proceeded from suspension cultures. The method separates foreground objects by the detection of their boundaries and then eliminates cell clumps and artefacts based on their different inside brightness and skeleton structures. Single cell objects are restored by the thickening of binary cell residues remaining after morphological operation of cleaning the holes under the cell chain masks. Experimental results of the segmentation for a series of BY-2 images as well as cell enumeration and segmentation errors are presented.
Keywords :
cellular biophysics; image segmentation; medical image processing; microscopes; BY-2 tobacco cell; binary cell residue; cell clumps; foreground object; microscope image; morphological operation; segmentation method; single cell object; skeleton structure; suspension culture; Brightness; Histograms; Image restoration; Image segmentation; Lighting; Microscopy; Morphological operations; Object detection; Shape; Skeleton; BY-2 tobacco cells; Elliptical Fourier Descriptors; dilatation; hit-miss operation; image processing; morphological operations; segmentation; skeletoning;
Conference_Titel :
Perspective Technologies and Methods in MEMS Design (MEMSTECH), 2010 Proceedings of VIth International Conference on
Conference_Location :
Lviv
Print_ISBN :
978-1-4244-7325-0
Electronic_ISBN :
978-966-2191-11-0