Title :
Double-helix microscopy for wide-field 3D single-molecule fluorescence imaging
Author :
Sharma, Ginni ; Pavani, Rama Prasanna Prasanna ; Quirin, Sean ; Piestun, Rafael
Author_Institution :
Dept. of Electr., Comput. & Energy Eng., Univ. of Colorado, Boulder, CO, USA
Abstract :
We present methods to improve the localization accuracy in wide-field 3D single-molecule double-helix microscopy. We analyze the optical efficiency of the system, the fundamental limit for 3D localization, the estimation algorithms, and polarization sensitive detection.
Keywords :
fluorescence; light polarisation; optical microscopy; 3D localization; double helix microscopy; estimation algorithms; localization accuracy; polarization sensitive detection; wide field 3D single molecule fluorescence imaging; DH-HEMTs; Data mining; Fluorescence; Focusing; Lenses; Microscopy; Optical imaging; Optical sensors; Polarization; Yield estimation;
Conference_Titel :
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-890-2
Electronic_ISBN :
978-1-55752-890-2
