DocumentCode
520239
Title
Internal quadratic stark effect results in color hue variations in fluorescent proteins with the same chromophore structure
Author
Drobizhev, M. ; Tillo, Shane ; Makarov, Nikolay S ; Rebane, A. ; Hughes, Thomas
Author_Institution
Dept. of Phys., Montana State Univ., Bozeman, MT, USA
fYear
2010
fDate
16-21 May 2010
Firstpage
1
Lastpage
2
Abstract
Genetically-encoded fluorescent proteins are widely used for bio-imaging. We employ two-photon absorption spectroscopy to show that their different hues can be explained by quadratic Stark effect due to variations of electric field within the protein.
Keywords
fluorescence; proteins; quantum confined Stark effect; bio-imaging; chromophore structure; color hue variations; fluorescent proteins; internal quadratic Stark effect; two-photon absorption spectroscopy; Absorption; Biological cells; Fluorescence; Frequency; Neuroscience; Physics; Polarization; Proteins; Spectroscopy; Stark effect;
fLanguage
English
Publisher
ieee
Conference_Titel
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location
San Jose, CA
Print_ISBN
978-1-55752-890-2
Electronic_ISBN
978-1-55752-890-2
Type
conf
Filename
5499808
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