Title :
Internal quadratic stark effect results in color hue variations in fluorescent proteins with the same chromophore structure
Author :
Drobizhev, M. ; Tillo, Shane ; Makarov, Nikolay S ; Rebane, A. ; Hughes, Thomas
Author_Institution :
Dept. of Phys., Montana State Univ., Bozeman, MT, USA
Abstract :
Genetically-encoded fluorescent proteins are widely used for bio-imaging. We employ two-photon absorption spectroscopy to show that their different hues can be explained by quadratic Stark effect due to variations of electric field within the protein.
Keywords :
fluorescence; proteins; quantum confined Stark effect; bio-imaging; chromophore structure; color hue variations; fluorescent proteins; internal quadratic Stark effect; two-photon absorption spectroscopy; Absorption; Biological cells; Fluorescence; Frequency; Neuroscience; Physics; Polarization; Proteins; Spectroscopy; Stark effect;
Conference_Titel :
Lasers and Electro-Optics (CLEO) and Quantum Electronics and Laser Science Conference (QELS), 2010 Conference on
Conference_Location :
San Jose, CA
Print_ISBN :
978-1-55752-890-2
Electronic_ISBN :
978-1-55752-890-2
