Expression of a chitinase gene from chaetomium cupreum in saccharomyces cerevisiae

Author

Zhang, Haiyan ; Ji, Hongfang ; Zhang, Lingwen

Author_Institution

Coll. of Life Sci., Henan Univ., Kaifeng, China

Volume

5

fYear

2010

fDate

16-18 Oct. 2010

Firstpage

2045

Lastpage

2048

Abstract

Chitinases perform a key role in defense mechanisms of biocontrol fungi against pathogenic fungi. A chitinase gene (designated Chi46) of C. cupreum which has a potential against a range of plant pathogens was amplified by RT-PCR and then integrated into the genomic DNA of pYES2 by insertion into a single site for recombination, yielding the recombinant pYES2/Chit46, which was used to transform to S. cerevisiae H158. Chitinase expression of recombinant was induced by galactose and repressed by glucose. The enzyme activity reached the peak expression after cultured for 48 h with pH 6.0 (maximal activity 1.96 units mL^-1).

Keywords

DNA; biochemistry; biocontrol; cellular biophysics; enzymes; genetics; genomics; microorganisms; molecular biophysics; sugar; Chaetomium cupreum; Chi46; RT-PCR; Saccharomyces cerevisiae; biocontrol fungi; chitinase expression; chitinase gene; defense mechanisms; enzyme activity; galactose; genomic DNA; glucose; pH 6.0; pathogenic fungi; plant pathogens; recombinant pYES2/Chit46; recombination; Biochemistry; Biological control systems; Chemicals; Pathogens; RNA; Sugar; Temperature measurement; Chaetomium cupreum; Expression of chitinase; Saccharomyces cerevisiae;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2010 3rd International Conference on

Conference_Location

Yantai

Print_ISBN

978-1-4244-6495-1

Type

conf

DOI

10.1109/BMEI.2010.5639501

Filename

5639501