Title :
A Robust Algorithm for Segmenting and Tracking Clustered Cells in Time-Lapse Fluorescent Microscopy
Author :
Tarnawski, W. ; Kurtcuoglu, V. ; Lorek, P. ; Bodych, M. ; Rotter, J. ; Muszkieta, M. ; Piwowar, L. ; Poulikakos, D. ; Majkowski, M. ; Ferrari, A.
Author_Institution :
Dept. of Syst. & Comput. Networks, Wroclaw Univ. of Technol., Wrocław, Poland
Abstract :
We present herein a robust algorithm for cell tracking in a sequence of time-lapse 2-D fluorescent microscopy images. Tracking is performed automatically via a multiphase active contours algorithm adapted to the segmentation of clustered nuclei with obscure boundaries. An ellipse fitting method is applied to avoid problems typically associated with clustered, overlapping, or dying cells, and to obtain more accurate segmentation and tracking results. We provide quantitative validation of results obtained with this new algorithm by comparing them to the results obtained from the established CellProfiler, MTrack2 (plugin for Fiji), and LSetCellTracker software.
Keywords :
biological techniques; biology computing; cellular biophysics; fluorescence; image sequences; optical microscopy; CellProfiler software; LSetCellTracker software; MTrack2 software; clustered cell segmentation; clustered cell tracking; clustered nuclei; ellipse fitting method; multiphase active contour algorithm; time-lapse 2-D fluorescent microscopy image sequence; Cell segmentation and tracking; ellipse fitting; fluorescent microscopy; multiphase active contours;
Journal_Title :
Biomedical and Health Informatics, IEEE Journal of
DOI :
10.1109/JBHI.2013.2262233
