Comparison of voltage-sensitive dye di-4-ANNEPS effects in isolated hearts of rat, guinea pig, and rabbit

Author

Fialova, Katerina ; Kolarova, Jana ; Provaznik, Ivo ; Novakova, Marie

Author_Institution

Dept. of Physiol., Masaryk Univ. Brno, Brno, Czech Republic

fYear

2010

fDate

26-29 Sept. 2010

Firstpage

565

Lastpage

568

Abstract

Voltage-sensitive dyes (VSDs) are used for recording of monophasic action potentials (MAPs) by optical method in excitable tissues. Their direct effects on the tissue are not completely elucidated. Previously we studied effects of VSD di-4-ANEPPS during staining and washout in Langendorff-perfused guinea pig and rabbit isolated hearts. However, most often used species in basic cardiology is rat. Therefore, we decided to compare the electrophysiological effects of VSD in isolated hearts of rabbit, guinea pig, and rat. Touch-less electrogram was recorded, the heart rate assessed and normalized. The type and incidence of arrhythmias were evaluated. Based on our results although the procedure of staining with the dye affects electrophysiological properties of the myocardium in all studied species - rat heart may be considered as the most suitable for electrophysiological studies using di-4-ANEPPS.

Keywords

biomedical measurement; diseases; electrocardiography; electromyography; patient diagnosis; Langendorff-perfused guinea pig; arrhythmia; electrophysiological effects; guinea; heart rate; monophasic action potentials; myocardium; pig; rabbit isolated hearts; rat; touch-less electrogram; voltage-sensitive dye; Cardiology; Heart rate; Myocardium; Optical fibers; Optical recording; Rabbits;

fLanguage

English

Publisher

ieee

Conference_Titel

Computing in Cardiology, 2010

Conference_Location

Belfast

ISSN

0276-6547

Print_ISBN

978-1-4244-7318-2

Type

conf

Filename

5738035