Title :
Comparison of various activation methods of Sepharose CL-4B to optimize the stability of immunoadsorbents
Author :
Ubrich, Nathalie ; Regnault, Véronique ; De Seyssel, Marc ; Rivat, Claude ; Hubert, Patrick ; Dellacherie, Edith
Author_Institution :
INSERM, Vandoeuvre-Les-Nancy, France
fDate :
Oct. 29 1992-Nov. 1 1992
Abstract :
During the use of chromatographic supports for the production of pure proteins or the cleansing of biological fluids by affinity chromatography, a leakage of ligands coupled to the matrix is generally observed. To study and minimize this one, a comparison of immunoadsorbents prepared with Sepharose CL-4B activated by various chemical reagents, and coupled to goat anti apoB polyclonal antibodies was performed.
Keywords :
adsorption; biochemistry; chromatography; molecular biophysics; proteins; Sepharose CL-4B; activation method; affinity chromatography; biological fluids; chemical reagents; cleansing; goat antiapoB polyclonal antibodies; immunoadsorbents; proteins; Ethanol; Immune system; Neodymium; Voltage control;
Conference_Titel :
Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE
Conference_Location :
Paris
Print_ISBN :
0-7803-0785-2
Electronic_ISBN :
0-7803-0816-6
DOI :
10.1109/IEMBS.1992.5760833
