Application of FT-IR spectroscopy in ultra-structural studies of bacteria

Fourier-transform infrared spectroscopy was used to examine bacterial characteristic structural differences under different incubation conditions (media). The data obtained with bacteria grown in liquid medium then plated on solid medium agree with those previously obtained using other techniques: increase in sugar level inside (reserve) and/or around the bacteria (slime). In particular, it was found that the transition from solid to liquid medium immediately induced the decrease of this sugar level. Whereas in the transition from liquid to solid medium, sugar level increase was slower to occur.