Title :
Homogeneous fluorescence polarization immunoassay for eicosanoids
Author :
Matt, M. ; Nicolas, A. ; Donner, M. ; Stoltz, J.F.
Author_Institution :
Fac. des Sci. Pharm. et Biol., Lab. de Chim. Anal., Univ. de Nancy, Nancy, France
fDate :
Oct. 29 1992-Nov. 1 1992
Abstract :
Homogeneous fluorescence polarization immunoassay (FPIA) was applied to the development of an immunoassay of prostaglandin E1 (PGE1) selected as a model of eicosanoids. PGE1 was derivatized with fluoresceinthiocarbamyl ethylenediamine and purified by thin layer chromatography (TLC). The purity was checked by high performance liquid chromatography (HPLC). A radioimmunoassay was realized to ensure of the immunocompetition of the tracer. After the determination of the concentration of antiserum by fluorescence polarization, the feasability of the immunoassay was shown.
Keywords :
bio-optics; chromatography; fluorescence; molecular biophysics; FPIA; antiserum; eicosanoids; fluoresceinthiocarbamyl ethylenediamine; high performance liquid chromatography; homogeneous fluorescence polarization immunoassay; immunocompetition; prostaglandin E1; purity; radioimmunoassay; thin layer chromatography; Anisotropic magnetoresistance; Electronics packaging; Fluorescence;
Conference_Titel :
Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE
Conference_Location :
Paris
Print_ISBN :
0-7803-0785-2
Electronic_ISBN :
0-7803-0816-6
DOI :
10.1109/IEMBS.1992.5760872
