Title :
A membrane capacitance measurement system for the study of exocytosis in secretory cells
Author :
Shaughnessy, Thomas J O ; Kim, Yong I.
Author_Institution :
Depts. of Biomed. Eng. & Neurology, Univ. of Virginia Health Sci. Center, Charlottesville, VA, USA
fDate :
Oct. 29 1992-Nov. 1 1992
Abstract :
A system for measuring exocytotic secretion in a single cell was developed utilizing an IBM compatible 386 PC. With sinusoidal excitation of the cells, a software based phase detection technique was implemented to monitor exocytosis-induced changes in membrane capacitance. The system is capable of monitoring exocytosis in response to either calcium or depolarizing voltage stimulation. The system has proven to be a useful tool in the study of changes in exocytosis produced by drugs and neurological disorders.
Keywords :
bioelectric potentials; biomedical measurement; biomembrane transport; calcium; capacitance measurement; drugs; medical disorders; medical signal processing; neurophysiology; Ca; IBM compatible 386 PC; depolarizing voltage stimulation; drugs; exocytosis; exocytotic secretion; membrane capacitance measurement system; neurological disorders; phase detection; secretory cells; sinusoidal cell excitation; Capacitance; Filtering theory; Monitoring; Noise measurement; Software; Timing; Voltage measurement;
Conference_Titel :
Engineering in Medicine and Biology Society, 1992 14th Annual International Conference of the IEEE
Conference_Location :
Paris
Print_ISBN :
0-7803-0785-2
Electronic_ISBN :
0-7803-0816-6
DOI :
10.1109/IEMBS.1992.5760874
