Batch-operation process of nuclear transplantation based on global field of view

Nuclear transplantation is one of key procedures in animal cloning. Traditional batch-operations of nuclear transplantation are manipulated by professional operators under the view of microscope and the operation process is too complicated and time consuming. This paper introduced NKTYMR601 Micro-manipulation system into nuclear transplantation and proposed a batch-operation process of nuclear transplantation based on global field of view and several key procedures including building of operation map based on global field of view, oocyte location in global field, sort based on operation order, relocation in local area, oocyte enucleation and donor cell injection were achieved. Finally, this operation process was applied in sheep somatic cell cloning and the results of several batches of experiment proved the validity of the batch-operation process of nuclear transplantation based on global field of view.