Title :
The analysis on fluorescence signals of the multi-channel DNA analyzer
Author :
Fang, Yi ; Wang, Liqiang ; Xu, Jin ; Wang, Pengpeng ; Yan, Huimin
Author_Institution :
State Key Lab. of Modern Opt. Instrum., Zhejiang Univ., Hangzhou, China
Abstract :
The original data generated by multi-channel DNA analyzer is a mixture in time domain of data of multiple channels and that of multiple spectra. Thus, it is necessary to preprocess the original data before the DNA sequence can be identified. In this paper, the processing procedure is proposed, including choosing data segment, deburring, generating four-color information, choosing channels, data filtering and removing baseline. The experiment results state that useful information can be well separated from original data. The noise is greatly reduced and signal peaks are clearer. And that will provide the basis for determining the type and position of bases.
Keywords :
DNA; bioinformatics; biological techniques; data analysis; fluorescence spectroscopy; molecular biophysics; signal processing; spectral analysis; DNA sequence; baseline removal; channel choosing; data filtering; data preprocessing; data segment choosing; data time domain; deburring; fluorescence signal analysis; four color information generation; multichannel DNA analyzer; multiple channels; multiple spectra; Computers; Educational institutions; Filtering; Noise; data filtering; deburring; fluorescence signals; four-color; removing baseline;
Conference_Titel :
Electronics and Optoelectronics (ICEOE), 2011 International Conference on
Conference_Location :
Dalian
Print_ISBN :
978-1-61284-275-2
DOI :
10.1109/ICEOE.2011.6013373
