Multi-channel and four-color laser induced fluorescence detection system and its application in DNA analysis equipment

Author

Wang, Pengpeng ; Shi, Yan ; Wang, Liqiang ; Fang, Yi ; Yan, Huimin

Author_Institution

State Key Lab. of Modern Opt. Instrum., Zhejiang Univ., Hangzhou, China

Volume

3

fYear

2011

fDate

29-31 July 2011

Abstract

A multi-channel and four-color fluorescence detection system was designed. It mainly consisted of a laser confocal scanning detection platform, an electrophoresis platform, an embedded control system and a data analysis processing system. A 16-channel capillary array, a scanning galvo and an f-θ lens were utilized to reach high-throughput capability. By switching filters and adjusting scanning speed, four-color function was achieved. The detection system was employed in a DNA experiment setup. Results indicate that the system is suitable for high-throughput DNA analysis equipment.

Keywords

DNA; biological techniques; biomedical measurement; data analysis; electrophoresis; fluorescence spectroscopy; galvanometers; laser applications in medicine; molecular biophysics; DNA analysis equipment; capillary array; data analysis processing system; electrophoresis platform; embedded control system; f-theta lens; four color function; fourcolor LIF detection system; high throughput DNA analysis; laser confocal scanning detection platform; laser induced fluorescence; multichannel LIF detection system; scanning galvanometer; Computers; DNA; Databases; Fluorescence; Lasers; Neodymium; DNA analysis; fluorescence detection; four-color; multi-channel;

fLanguage

English

Publisher

ieee

Conference_Titel

Electronics and Optoelectronics (ICEOE), 2011 International Conference on

Conference_Location

Dalian

Print_ISBN

978-1-61284-275-2

Type

conf

DOI

10.1109/ICEOE.2011.6013374

Filename

6013374