A LED-induced confocal fluorescence detection system for quantitative PCR instruments

Fluorescent quantitative PCR (Q-PCR) technology has been an active research area since it has excellent performance of fluorescence signal detection and the ability of quantitative analysis for initial DNA template evaluation. A compact design of fluorescence detection system based on enhanced confocal type is developed. This paper basically focuses on the features of each component of the system and the determination of device lectotype. Quantified results of the experimental test show a much lower background noise level, and 8-tube scanning of double diluted rhodamine B solutions (minimal concentration: 1.21 ppm) ulteriorly demonstrates the effectiveness of the instrument we designed.