Development and primary application of the SYBR Green I RT-qPCR assay for the detection of the transmissible gastroenteritis virus (TGEV) S gene

Author

Gu, Jiangping ; Yue, Xiuwei ; Yuan, Congli ; Cui, Li ; Hua, Xiuguo ; Yang, Zhibiao ; Li, Caiying ; Xing, Rui

Author_Institution

Shanghai Key Lab. of Veterinary Biotechnol., Shanghai Jiaotong Univ., Shanghai, China

Volume

3

fYear

2011

fDate

15-17 Oct. 2011

Firstpage

1412

Lastpage

1415

Abstract

This study established a SYBR Green I real-time quantitative PCR detection assay (RT-qPCR) for the detection of the porcine transmissible gastroenteritis virus (TGEV) using specific primers designed to amplify the highly conserved porcine TGEV S gene sequence. The threshold cycle (Ct) and the log plasmid copy numbers had a good linear relationship with an efficiency of 1.05 (R² = 0.999). The advantages of utilizing this approach for the rapid detection of TGEV include excellent sensitivity, reproducibility, and low cost. Ninety-six porcine fecal samples were tested in this study, and 7 samples more than PCR assay were detected by this assay.

Keywords

biomedical engineering; genomics; microorganisms; SYBR Green I RT-qPCR assay; TGEV S gene detection; log plasmid copy numbers; realtime quantitative PCR detection assay; threshold cycle; transmissible gastroenteritis virus detection; Diseases; Laboratories; Probes; Proteins; Real time systems; Sensitivity; Strain; RT-qPCR; S gene; SYBR Green I; TGEV;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2011 4th International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-9351-7

Type

conf

DOI

10.1109/BMEI.2011.6098527

Filename

6098527