Title :
Separation and characterization of soyasaponin-β-glucosidase from Aspergillus sp.48
Author :
Tian, Jing ; Hong, Zhenyu ; Xu, Longquan ; Zhai, Bin
Author_Institution :
Modern Technol. Branch, Dalian Polytech. Univ., Dalian, China
Abstract :
The enzyme which hydrolyzes soyasaponin to lower sugar soyasaponin was isolated from Aspergillus sp.48s. And it was purified by the method of gradient protein chromatography of middle pressure with biologic chromatography system. In order to determination the purity and the molecular weight of the enzyme produced by Aspergillus sp.48s, the method of SDS-polyacrylamide gel electrophoresis was used. The molecular weight was about 34 kDa. The optimum pH of soyasaponin-β-glucosidase produced from sp.48s was 5.0, and the optimum temperature was 40°C. The enzyme was comparatively stable in the pH range from 3.0 to 7.0 and from 30°C to 60°C.
Keywords :
biochemistry; bioelectric phenomena; biological techniques; biothermics; chromatography; electrophoresis; enzymes; microorganisms; molecular biophysics; molecular weight; pH; purification; separation; Aspergillus sp.48; SDS-polyacrylamide gel electrophoresis; biologic chromatography system; enzyme; gradient protein chromatography; molecular weight; optimum temperature; pH; purification; purity; soyasaponin-β-glucosidase separation; temperature 30 degC to 60 degC; Educational institutions; Proteins; Purification; Stability analysis; Sugar; β-glucosidase; purification; soyasaponin;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2011 4th International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-9351-7
DOI :
10.1109/BMEI.2011.6098580
