Title :
Improved detection limits of bacterial endotoxins using new type of planar interdigital sensors
Author :
Syaifudin, A R Mohd ; Zia, Asif I. ; Mukhopadhyay, S.C. ; Yu, P.L. ; Gooneratne, Chinthaka P. ; Kosel, Jurgen
Author_Institution :
Sch. of Eng. & Adv. Technol., Massey Univ., Palmerston North, New Zealand
Abstract :
New types of planar interdigital sensors were fabricated by photolithography and etching techniques on a Silicon/Silicon Dioxide (Si/SiO2) wafer (single side polished). The sensors were then coated with APTES (3-aminopropyltrietoxysilane) a cross linker used to bind Polymyxin B (PmB) molecules on electrodes surface. PmB is an antimicrobial peptide produced by the Gram-positive bacterium-Bacillus which has specific binding properties to Lipopolysaccharide (LPS). This paper will discuss the fabrication process, coating and immobilization procedures and analysis of sensors´ performance based on Impedance Spectroscopy method. The sensor sensitivity was compared to standard ToxinSensor Chromogenic LAL Endotoxin Assay Kit for verification.
Keywords :
biological techniques; cellular biophysics; chemical sensors; coating techniques; electrodes; etching; interdigital transducers; microorganisms; molecular biophysics; organic compounds; photolithography; 3-aminopropyltrietoxysilane; APTES; Gram-positive bacterium-Bacillus; LPS; PmB molecules; Si-SiO2; antimicrobial peptide; bacterial endotoxin detection limit; bind polymyxin B; coating process; electrodes surface; etching technique; fabrication process; immobilization procedure; impedance spectroscopy method; lipopolysaccharide; photolithography; planar interdigital sensor; Electrodes; Gold; Impedance; Resists; Sensitivity; Sensors; Silicon;
Conference_Titel :
Sensors, 2012 IEEE
Conference_Location :
Taipei
Print_ISBN :
978-1-4577-1766-6
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2012.6411193
