2D-SPR biosensor detects the intracellular signal transduction in PC 12 cells at single cell level

Conventional techniques for intracellular signal transduction in living cells can be limited by the need for exogenous labels, time and labor-intensive protocols, as well as by poor sensitivity levels. In this study, 2D-SPR imager was applied to real-time monitoring of intracellular signal transduction at single cell level upon stimulation with carbachol. The reflection intensity change response was suppressed by atropine, D-tubocurarine and staurosporine, respectively. Our results demonstrated that the mammalian cell-based 2D-SPR sensor might develop a pathway to non-labeled, simple, rapid, highly sensitive and multi-detectable biodetection for various chemicals and greatly facilitate high-throughput drug screening, study of molecular biology and so on.