Microfluidic device optimization for cell growth

Intensive biological research has been directed towards why and how cells respond to a dynamic environment. Microfabrication has emerged as crucial tool to capture dynamic response. Our current study describes the approaches taken to fabricate, optimize, and validate a biocompatible device. Our experimental observations revealed that attempt to increase the channel depth induced surface roughness, which affects the device characteristics. Rough surface may not facilitate smooth pattern transfer to PDMS and more or less affects opacity of device. Thus Multiobjective optimization (MOO) helps us to choose the right combination of parameters to develop a device suitable for cell growth.