DocumentCode :
605650
Title :
Quantitative stem cell imaging with magnetic particle imaging
Author :
Bo Zheng ; Vazin, T. ; Yang, Weiguo ; Goodwill, P.W. ; Saritas, E.U. ; Croft, L.R. ; Schaffer, David V. ; Conolly, S.M.
Author_Institution :
Bioeng., UC Berkeley, Berkeley, CA, USA
fYear :
2013
fDate :
23-24 March 2013
Firstpage :
1
Lastpage :
1
Abstract :
MPI stem cell imaging - sensitivity and linearity: 9 pelletized, labeled hESC-derived cell populations (Fig. 2A) with varying cell numbers were imaged successively in the FFL imager. Fig. 2B shows the maximum image intensity from each reconstructed cell pellet image as a function of cell number. When a linear fit was applied, we found a strong linear correlation between MPI signal intensity and cell number, with R2 > 0.99. Additionally, the detection sensitivity of the projection MPI system was found to be slightly over 104 cells. We found no detectable MPI signal from unlabeled cell populations. Mice imaging: A MPI image of postmortem cell injections containing 4×105 and 6×105 is shown in Fig. 2C. The MPI image shows excellent contrast with minimal tissue effects. Additionally, the ratio of total MPI signal between the 6×105 and 4×105 injection regions was found to be 1.5, as expected.
Keywords :
biomagnetism; cellular biophysics; image reconstruction; magnetic particles; medical image processing; FFL imager; MPI image; MPI signal intensity; MPI stem cell imaging; cell number; detectable MPI signal; detection sensitivity; hESC-derived cell populations; magnetic particle imaging; maximum image intensity; mice imaging; minimal tissue effects; postmortem cell injections; projection MPI system; quantitative stem cell imaging; reconstructed cell pellet image; strong linear correlation; unlabeled cell populations; Imaging; Magnetic particles; Mice; Sensitivity; Sociology; Statistics; Stem cells;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Magnetic Particle Imaging (IWMPI), 2013 International Workshop on
Conference_Location :
Berkeley, CA
Print_ISBN :
978-1-4673-5520-9
Electronic_ISBN :
978-1-4673-5521-6
Type :
conf
DOI :
10.1109/IWMPI.2013.6528323
Filename :
6528323
Link To Document :
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