Title :
In vivo measurement of local biomarker concentrations
Author :
Xiaojuan Zhang ; Reeves, D.B. ; Perreard, I.M. ; Kett, W.C. ; Griswold, K.E. ; Gimi, B. ; Weaver, John B.
Author_Institution :
Dept. of Radiol., Dartmouth Coll., Hanover, NH, USA
Abstract :
The reaction has been explored in vitro in solution and the results for a DNA target are provided in Fig. 2. The 24 base pair DNA target can be found with 100 pM sensitivity. Shorter 20 base pair DNA target molecules bound the NPs together with less affinity so the sensitivity is only 2 nM. DNA molecules with incorrect sequencing produced no change in the MSB signal. Thrombin can also be measured with 4 nM sensitivity and streptavidin with 150 pM sensitivity. These proof of concept experiments demonstrate that the method is feasible for hormone concentrations.
Keywords :
DNA; biochemistry; biomagnetism; enzymes; magnetic particles; molecular biophysics; molecular configurations; nanobiotechnology; nanoparticles; Brownian motion signal; MSB signal; base pair DNA target molecules; hormone concentrations; in vivo measurement; local biomarker concentrations; magnetic nanoparticle spectroscopy; streptavidin; thrombin; DNA; Educational institutions; Nanoparticles; Physics; Sensitivity; Temperature measurement; Weaving;
Conference_Titel :
Magnetic Particle Imaging (IWMPI), 2013 International Workshop on
Conference_Location :
Berkeley, CA
Print_ISBN :
978-1-4673-5520-9
Electronic_ISBN :
978-1-4673-5521-6
DOI :
10.1109/IWMPI.2013.6528364
