Title :
Role of biofunctionalization and tracer cross-linking in magnetic particle spectrometry
Author :
Arami, H. ; Ferguson, Richard Matthew ; Khandhar, A.P. ; Tomitaka, A. ; Krishnan, Kannan M.
Author_Institution :
Dept. of Mater. Sci., Univ. of Washington, Seattle, WA, USA
Abstract :
In this paper, we present a ligand exchange and PEGylation approach for developing monodisperse MPI tracers (core size ~ 20nm) with the required reactive -NH2 and/or -SH functional groups on their surface. First, free carboxyl groups were introduced on the surface of the NPs through a silanization reaction. Then, a carbodiimide reagent (DCC) was used to bond the amine groups of the NH2-PEG-NH2 or NH2-PEG-SH molecules to these carboxyl groups.
Keywords :
biomagnetism; exchange interactions (electron); magnetic particles; molecular biophysics; nanomagnetics; nanoparticles; proteins; spectrochemical analysis; PEGylation approach; amine groups; biofunctionalization; carbodiimide reagent; carboxyl groups; core size; free carboxyl groups; ligand exchange; magnetic particle spectrometry; monodisperse MPI tracers; reactive NH2 functional groups; reactive SH functional groups; silanization reaction; tracer cross-linking; Cancer; Hydrodynamics; Imaging; Magnetic cores; Magnetic particles; Nanobioscience; Spectroscopy;
Conference_Titel :
Magnetic Particle Imaging (IWMPI), 2013 International Workshop on
Conference_Location :
Berkeley, CA
Print_ISBN :
978-1-4673-5520-9
Electronic_ISBN :
978-1-4673-5521-6
DOI :
10.1109/IWMPI.2013.6528378
