Immobilization of BSA on the polymethacrylic acid

Author

Fang Lv ; Xiu-jie Liu ; Kai-yue Chi ; Rong-ji Dai ; Yu-lin Deng

Author_Institution

Sch. of Life Sci., Beijing Inst. of Technol., Beijing, China

fYear

2013

fDate

25-28 May 2013

Firstpage

501

Lastpage

503

Abstract

N, N´-Methylenebisacrylamide was used as the crosslinking agent to prepare the polymethacrylic acid and its carboxyl groups were modified by N-(3-Dimethy-laminopropyl)-N´-ethyl carbodiimide hydrochloride and N-Hydroxy succinimide. The ability of immobilizing BSA and the influence of reacting time of carrier were studied. The immobilization amount and the optimal reacting time were 2100mg/g and 90 minutes respectively. The carrier was characterized by infrared detection. The results indicated that the polymethacrylicacid possessed good performance and the ability to immobilized BSA was enhanced after the modification of EDC-NHS.

Keywords

biochemistry; infrared spectra; molecular biophysics; polymers; proteins; BSA immobilization; EDC-NHS; N,N´-methylenebisacrylamide; N-(3-dimethy-laminopropyl)-N´-ethyl carbodiimide hydrochloride; N-hydroxy succinimide; carboxyl groups; carrier reacting time; crosslinking agent; infrared detection; polymethacrylic acid; Adsorption; Chemicals; Electron tubes; Plastics; Proteins; immobilized protein; modified carrier; polymethacrylicacid;

fLanguage

English

Publisher

ieee

Conference_Titel

Complex Medical Engineering (CME), 2013 ICME International Conference on

Conference_Location

Beijing

Print_ISBN

978-1-4673-2970-5

Type

conf

DOI

10.1109/ICCME.2013.6548300

Filename

6548300