Different protein profile in amniotic fluid with nervous system malformations by SELDI-TOF-MS technology

To detect the distinct proteins in amniotic fluid between nervous system malformations fetuses and normal fetuses. Surface-enhanced laser desorption-ionization/time-of-flight mass spectrometry was used to characterize amniotic fluid peptides in amniotic fluid between nervous system malformations fetuses and normal fetuses. Protein chips were used to characterize amniotic fluid peptides in amniotic fluid. Protein chips were examined in protein reader, the protein profiling was collected by proteinchip software 3.1 and analyzed by Biomarker Wizard software. Nine distinct proteins were identified in amniotic fluid between nervous system malformations fetuses and normal fetuses. The results suggest that there are distinct proteins in protein profiling of amniotic fluid between nervous system malformations fetuses and normal fetuses.