Evaluation of Antioxidant Activity of Natural and Synthetic Compounds Against LDL Oxidation Using CNT Electrode

Author

Ohkawa, Futaba ; Takeda, Shigeki ; Shu-Ping Hui ; Sakurai, Takayasu ; Jin, Seongwook ; Fuda, Hirotoshi ; Sueoka, Kazuhisa ; Chiba, Hidetoshi

Author_Institution

Dept. of Health Sci., Hokkiado Univ., Sapporo, Japan

Volume

14

Issue

2

fYear

2014

fDate

Feb. 2014

Firstpage

532

Lastpage

537

Abstract

In this paper, a novel method for evaluating antioxidant activity against low density lipoprotein (LDL) oxidation (anti-LDLox) using a carbon nanotube (CNT) based electrode was investigated. Although ORAC and DPPH are known methods to evaluate antioxidant activity, they do not directly reflect the capacities of anti-LDLox. A method has been reported for measuring oxidized LDL using a CNT electrode. We evaluated anti-LDLox by measuring the amount of oxidized LDL using the electrode after incubation of LDL with antioxidants during copper-mediated oxidation. Thiobarbituric acid reactive substances (TBARS) were also determined for comparison with the CNT electrode. There was a correlation of the CNT electrode with the TBARS and the ORAC, whereas no correlation was found between the CNT electrode and the DPPH. We demonstrate here that a CNT electrode method has a potential for the determination of the antioxidant activity of various natural and synthetic compounds by detecting oxidized LDL.

Keywords

biological techniques; carbon nanotubes; electrochemical electrodes; electrochemical sensors; proteins; LDL oxidation; TBARS; Thiobarbituric acid reactive substance; antioxidant activity; carbon nanotube electrode; copper mediated oxidation; low density lipoprotein oxidation; natural compound; oxidized LDL measurement; synthetic compound; Correlation; Electric potential; Electrodes; Lipidomics; Materials; Oxidation; Sensors; Low-density lipoprotein; bioelectric phenomena; biotechnology;

fLanguage

English

Journal_Title

Sensors Journal, IEEE

Publisher

ieee

ISSN

1530-437X

Type

jour

DOI

10.1109/JSEN.2013.2285408

Filename

6644270