Oviduct -mimetic chip for sperm separation and oocyte manipulation to enhance the probability of fertilization for oligozoospermia patient

This study presents an imitation of oviduct microfluidic dielectrophoresis chip system. In this research, ICR mouse´s oocyte trapped by insulator-structure microchannel of a dielectrophoresis system will be shown. In order to reduce the impact and destruction of cells, this study adopts dielectrophoresis force to manipulate the cell. Imitation of In Vitro Fertilization, the natural fertilization of sperm and oocytes in the microfluidic channel, the collision frequency between sperms and oocyte plays the success key role of the IVF. The numerical simulation software, CFDRC, was used to reveal the position of high and low electric field distribution in microchannel. The positive dielectrophoresis response of oocyte was exhibited with the frequency at 1 MHz, the oocyte will move to the region with high electric field density The pattern of insulating structure was fabricated by SU8-3050 to generate non-uniform electric field to trap oocyte with positive dielectrophoresis. Finally, the positive DEP was used to drive the position of cells in the microchannel structures.