Label-free separation and sorting of human monocytes and T-cells by electrowetting and dielectrophoresis

Author

Cheng-Yeh Huang ; Min-Yu Chiang ; Shih-Kang Fan ; Ghaemmaghami, Amir M. ; Wensyang Hsu

Author_Institution

Dept. of Mech. Eng., Nat. Chiao Tung Univ., Hsinchu, Taiwan

fYear

2013

fDate

7-10 April 2013

Firstpage

873

Lastpage

876

Abstract

We report a label-free separation and sorting approach based on electrowetting-on-dielectric (EWOD) and dielectrophoresis (DEP) to manipulate human monocyte (THP-1) and T-cell (Jurkat) lines in droplets without any impact on cell viability. In addition to separating cells, this device provides a platform to study cell-cell interactions and migration before the separation.

Keywords

bioelectric phenomena; biological techniques; cell motility; dielectric materials; drops; electrophoresis; wetting; DEP; EWOD; Jurkat line manipulation; THP-1 monocyte manipulation; cell migration; cell viability impact; cell-cell interaction; dielectrophoresis; droplet; electrowetting-on-dielectric; human monocyte label-free separation; human monocyte sorting; human t-cell separation; human t-cell sorting; Conductivity; Dielectrophoresis; Electrodes; Sorting; Strips; Voltage measurement; T-cell; dielectrophoresis; label-free separation; monocyte;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2013 8th IEEE International Conference on

Conference_Location

Suzhou

Electronic_ISBN

978-1-4673-6351-8

Type

conf

DOI

10.1109/NEMS.2013.6559863

Filename

6559863