Title :
Isolation of collagen type II from bovine tracheal cartilage
Author :
Soto-Herrera, M.E. ; Casadiegos, S.A. ; Fontanilla, M.R.
Author_Institution :
Grupo de Trabajo en Ing. de Tejidos, Univ. Nac. de Colombia, Manizales, Colombia
fDate :
April 29 2013-May 4 2013
Abstract :
Currently, the treatment of articular cartilage defects results in tissue repair that leads to osteoarthritis [1]. Autologous chondrocyte implantation (ACI) is a technique based in obtaining autogenous chondrocytes from a cartilage biopsy. After culturing, the cells are seeded onto biodegradable scaffolds that provide the environment required for growing them. The grown chondrocytes replace and remodel the scaffold to produce artificial cartilage used for grafting. Indeed, surgical procedures based on artificial tissue have been already marketed in USA and Europe (Carticel®, BioCart). Unfortunately, the clinical implementation of ACI has been limited by its high cost: Colombian patients who need ACI must travel to USA, Italy or Israel where authorized cell processing facilities are located. Therefore, there is a need for the development of in-site production of tissue engineered cartilage to provide patients of all socioeconomic status with ACI at a reasonable cost. A commonly biomaterial used to manufacture scaffolds for engineering artificial cartilage is collagen type II, a major component of the cartilage extracellular matrix. In this work scaffolds were produced using pure collagen type II isolated from bovine trachea. The microstructure of them was also studied.
Keywords :
biodegradable materials; biological tissues; biomedical materials; biomembranes; cellular biophysics; diseases; molecular biophysics; proteins; tissue engineering; Colombian patient; Europe; Israel; Italy; USA; articular cartilage defect treatment; artificial cartilage engineering; autologous chondrocyte implantation; biodegradable scaffold; biomaterial; bovine tracheal cartilage; cartilage biopsy; cartilage extracellular matrix component; cell culture; cell processing facility; cell seeding; collagen type II; osteoarthritis; surgical procedure; tissue repair; Bovine; Conferences; Couplings; Electronic mail; Maintenance engineering; Osteoarthritis; Arthritis; biodegradable materials; biomedical materials; biomembranes; proteins; regeneration engineering; tissue engineering;
Conference_Titel :
Health Care Exchanges (PAHCE), 2013 Pan American
Conference_Location :
Medellin
Print_ISBN :
978-1-4673-6254-2
DOI :
10.1109/PAHCE.2013.6568331