Laminar Optical Tomography Applied to Reporter Genes Imaging in Engineered Tissue Constructs

Author

Ozturk, Mehmet S. ; Lee, Vivian K. ; Lingling Zhao ; Guohao Dai ; Intes, Xavier

Author_Institution

Biomed. Eng. Dept., Rensselaer Polytech. Inst., Troy, NY, USA

fYear

2013

fDate

5-7 April 2013

Firstpage

129

Lastpage

130

Abstract

Fluorescence imaging is the main method in assessing tissue engineered constructs. However, with the drive to develop thick engineered constructs, it is imperative to develop new fluorescence based techniques that can image beyond the depth capabilities of current microscopic techniques. Here we report the use of Laminar Optical Tomography (LOT) to image in 3D vascular channels, formed by GFP and mCherry carrier cells, in a thick printed constructs enclosed in a perfusion chamber. Results show the potential of LOT for locating and monitoring transfected cells in thick tissue with spatial accuracy.

Keywords

biological tissues; biomedical materials; biomedical optical imaging; cellular biophysics; fluorescence; genetics; optical tomography; tissue engineering; 3D vascular channel; GFP; LOT potential; depth capability; fluorescence based technique; fluorescence imaging; laminar optical tomography; mCherry carrier cells; microscopic technique; perfusion chamber; reporter gene imaging; thick engineered construct; thick printed construct; tissue engineered construct; transfected cell locating; transfected cell monitoring; Biomedical optical imaging; Fluorescence; Microscopy; Optical imaging; Optical microscopy; Tomography; 3D reconstruction; mesoscopic; reporter genes; tissue engineering; tomographic;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2013 39th Annual Northeast

Conference_Location

Syracuse, NY

ISSN

2160-7001

Print_ISBN

978-1-4673-4928-4

Type

conf

DOI

10.1109/NEBEC.2013.106

Filename

6574391