Title :
Fabricating Consistently Sized Chitosan-Core NanoBioSensors
Author :
Dilai, Khrystyna ; Chambers, Kathryn E. ; Clements, Danielle E. ; Bankich, Patrick J. ; Robinson, Charles J.
Author_Institution :
Dept. of Chem. & Biomol. Eng., Clarkson Univ., Potsdam, NY, USA
Abstract :
Doubly labeled chitosan-TPP nanosensors provide a means of studying the effect of nitric oxide and glutamate excitotoxicity in the brain. In order to construct these nanosensors for use in vivo, it is necessary to develop a procedure that results in nanosensor cores of a consistent size. The goal of this project was to investigate various procedures and study the impact of solution temperature, pH, chitosan to TPP ratio, reagent concentration, sonication, and centrifugation on particle size. The optimal procedure involves combining aqueous chitosan and TPP in a 3:1 ratio and stirring at room temperature in 3.33% Acetic acid solution.
Keywords :
biosensors; chemical sensors; nanofabrication; nanomedicine; nanoparticles; nanosensors; nitrogen compounds; pH; polymers; sodium compounds; temperature; toxicology; NO; Na5P3O10; acetic acid solution; aqueous chitosan-TPP combination; brain; centrifugation effect; chitosan-TPP ratio effect; consistent nanosensor core size; consistently sized chitosan-core nanobiosensor fabrication; doubly labeled chitosan-TPP nanosensor; glutamate excitotoxicity; nanosensor construction; nitric oxide effect; pH effect; particle size; reagent concentration effect; solution temperature effect; sonication effect; temperature 293 K to 298 K; Biomedical engineering; Brain; Chemicals; Fluorescence; Nanobioscience; Nanoparticles; Sensors; Biosensor; Chitosan Nanoparticle; Ionic Gelation; Nitric Oxide;
Conference_Titel :
Bioengineering Conference (NEBEC), 2013 39th Annual Northeast
Conference_Location :
Syracuse, NY
Print_ISBN :
978-1-4673-4928-4
DOI :
10.1109/NEBEC.2013.95
