Fabricating Consistently Sized Chitosan-Core NanoBioSensors

Author

Dilai, Khrystyna ; Chambers, Kathryn E. ; Clements, Danielle E. ; Bankich, Patrick J. ; Robinson, Charles J.

Author_Institution

Dept. of Chem. & Biomol. Eng., Clarkson Univ., Potsdam, NY, USA

fYear

2013

fDate

5-7 April 2013

Firstpage

279

Lastpage

280

Abstract

Doubly labeled chitosan-TPP nanosensors provide a means of studying the effect of nitric oxide and glutamate excitotoxicity in the brain. In order to construct these nanosensors for use in vivo, it is necessary to develop a procedure that results in nanosensor cores of a consistent size. The goal of this project was to investigate various procedures and study the impact of solution temperature, pH, chitosan to TPP ratio, reagent concentration, sonication, and centrifugation on particle size. The optimal procedure involves combining aqueous chitosan and TPP in a 3:1 ratio and stirring at room temperature in 3.33% Acetic acid solution.

Keywords

biosensors; chemical sensors; nanofabrication; nanomedicine; nanoparticles; nanosensors; nitrogen compounds; pH; polymers; sodium compounds; temperature; toxicology; NO; Na₅P₃O₁₀; acetic acid solution; aqueous chitosan-TPP combination; brain; centrifugation effect; chitosan-TPP ratio effect; consistent nanosensor core size; consistently sized chitosan-core nanobiosensor fabrication; doubly labeled chitosan-TPP nanosensor; glutamate excitotoxicity; nanosensor construction; nitric oxide effect; pH effect; particle size; reagent concentration effect; solution temperature effect; sonication effect; temperature 293 K to 298 K; Biomedical engineering; Brain; Chemicals; Fluorescence; Nanobioscience; Nanoparticles; Sensors; Biosensor; Chitosan Nanoparticle; Ionic Gelation; Nitric Oxide;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioengineering Conference (NEBEC), 2013 39th Annual Northeast

Conference_Location

Syracuse, NY

ISSN

2160-7001

Print_ISBN

978-1-4673-4928-4

Type

conf

DOI

10.1109/NEBEC.2013.95

Filename

6574467