Optical mapping of optically paced embryonic hearts

Conduction maps of early embryonic hearts with optical mapping point to heterogeneous conduction patterns that quickly evolve over time. In adult hearts, electrical pacing is utilized to determine the anisotropy of the conduction patterns and the susceptibility of the tissue to arrhythmias. However, studying electrophysiology in developing hearts is limited due to their size. Electrical pacing creates an electrical artifact that obscures recordings from the entirety of early embryonic hearts. In this study, optical point stimulation using a 1440-nm near-infrared diode laser with a 12-μm diameter beam waist was used to pace embryonic quail hearts. Electrical activity was recorded across the surface of the embryonic hearts by high resolution optical mapping using di-4-ANEPPS and cytochalasin D. While there were no electrical artifacts produced by the optical point stimulation, an optical artifact due to thermal lensing did obscure the optical mapping near the point of stimulation. The optical artifact can be minimized by optimizing the stimulation parameters to minimize the energy deposited and can be further reduced by signal processing. Despite the presence of the optical artifact, the electrical activity over the majority of the heart can be obtained.